May 04 2011

Done with finals!

Published by under Uncategorized

I am done with finals! This semester has been a BLAST though…hehehe.  I am excited to go to the phage conference in June, but I will miss my fellow classmates :(

Comments Off

Apr 29 2011

Research Project

Published by under Uncategorized

As the semester comes to a close, I am working on my final research project proposal.  This has been a more difficult paper to compose than initially expected, however, and has required quite a bit of organization of my thoughts as well as the use of logic in order to communicate my ideas to the reader.  I’ve also noticed the amount of work that comes with citing the different sources used and how integral these can be when certain points are made.  What’s been hardest of all is finding all the different patterns associated with phage and attempting to blend these into one contiguous idea without drawing conclusions that are too far-fetched or irrelevant to the overall idea that I am trying to present.

Comments Off

Apr 29 2011

Snowingggggg

Published by under Uncategorized

It’s snowing outside… but on a brighter note, i’m almost done with my proposal. I ran my PCR and it showed that the tape measure protein gene is indeed present in my phage. Yay!! Also, we’re having a phage party today! It’s time to graduate!

Comments Off

Apr 27 2011

Update

Published by under Uncategorized

Hey guys,
Sadly, this may be the last time I have to update you. I was able to isolate my plasmid, despite being really freaking ADD and accidentally adding like 350x as much buffer as I should have…..whoops. But it still showed up on a gel when I doubled the samples run in the wells…so all is good (I think). Regardless of this fact, I think my project is going to have to be halted here for the semester, since this is our last week of classes. So, I’m going to take my results up to this point and write up my proposal paper for the project and then be done for the summer!
Have a good summer!
Peace

Comments Off

Apr 25 2011

A Rainy Phage-Phree Monday…

Published by under Uncategorized

It’s a Monday and we’re not in lab, hooray! I mean..waaaah. But in all seriousness, there’s only one more phage lab left for the year..and it’s going to be a party, we will be PHAGE GRADUATES! Definitely something to celebrate. We’ve accomplished a lot this year, between isolating all of our own phages, to annotating GUmbie’s genome, to showing off our results at a real scientific conference. It’s been phun going through all of this as one small phage phamily. I’m interested in getting to know the next generation of phagers so they can continue our legacy!

Comments Off

Apr 21 2011

Phriendly Phage Pholks

Published by under Uncategorized

What a great day of phage presentations at the Spokane Intercollegiate Research Conference! I am proud of you all — for your hard work, your dedication to excellence, and your enthusiasm when you tell others about your research. Good job!

Comments Off

Apr 19 2011

SIRC

Published by under Uncategorized

Participating in the Spokane Intercollegiate Research Conference this weekend was a lot of fun.  Sam Gordon, Joe Ryan, and I had the pleasure of presenting a poster examining the relationship between Gumbie’s structural and non-structural genes and those of other F1 cluster phage, along with an analysis of the novel gene 48.

Comments Off

Apr 19 2011

results?

Published by under Uncategorized

So I plated the infected smeg every two minutes over an hour time span and all of the plates ended up with plaques meaning they where all still phage sensetive. Because of this I decided to switch up my experiment and plate 24 and 48 hours after infection and we’ll see if it developes resistance.

Comments Off

Apr 18 2011

Blogging on Mondayyyyyy!!!!

Published by under Uncategorized

Today is the last Monday lab of the year! Today I am running PCR to determine whether the tapemeasure protein gene is present in my phage since it is present in all the other E cluster phages. The SIRC conference on Saturday was super cool. There were a lot of interesting posters that students had there and a lot of people asked my group about our poster too. One guy even gave me a high five because he thought our project was so fascinating!

Comments Off

Apr 18 2011

Independent Project Preliminary Results

Published by under Uncategorized

It turns out that altering the concentration of smeg used for infection does not alter plaque morphology. Rather, increasing the concentration merely causes an increase in plaques while decreasing concentration causes a decrease in the number of plaques. The size of all plaques observed was approximately the same, and plaques remained clear with a surrounding turbid ring. With those results in hand, it’s research proposal time.

Comments Off

Apr 18 2011

Done with SIRC

Published by under Uncategorized

Well, SIRC was this weekend and me, Tara, Erinleigh, and Chelsea gave our presentations. Not that many people were there in the beginning of my presentation because I went first, but then people really started to show up! I got a lot of questions about my PCR and Restriction Digest from the Whitworth students. One of the questions I didn’t know how to answer, so I looked over at Dr. Poxleitner and she helped me (thankfully). There was one other group of presenters who actually didn’t know that they were providing an oral presentation that showed up. The talked about fecal matter coliforms and the degradation of bacteria within the fecal matter as they metabolize. I thought it was a fairly interestig topic to say the least. I wish I could have gone to the poster session after the fact, though I had to come back to school and continue research for the chemistry department. Overall, the conference was a great experience, and I am so glad my friends were there to support me. :)

Comments Off

Apr 18 2011

Conference Phun!

Published by under Uncategorized

Going to this saturday’s Spokane Intercollegiate Research Conference (SIRC) was an interesting experience! The talk I performed on the isolation & cluster typing of GUmbie went better than I had anticipated, it was actually kind of fun. And then I attended the poster session with my group including Graciela, Riley, and Alexis. There were two biology professors who were especially interested in our project of isolating and characterizing our novel phages. The best part was probably that our class stood out thanks to our awesome bright orange HHMI research shirts! It was nice being at a conference where everyone has a little bit of a nerd in them : ) Hoping I’ll have more opportunities like this in my future.

Comments Off

Apr 18 2011

Conference Success

Published by under Uncategorized

The conference last weekend at Whitworth was quite (whit)worthwhile in my opinion. It was interesting to find out about other people’s undergraduate research and ask questions about their projects. While many of the people there were not trained biologists, it seemed like we were able to get our ideas across to people who were interested in our project.

Comments Off

Apr 18 2011

LAST MONDAY!

Published by under Uncategorized

Today is the last Monday of the semester!! Conference was good this past weekend! Good job to Sammy, Tara, Erinleigh, and Chelsea!! The poster session was interesting and we stood out with our orange shirts! Now it’s time to keep working on my experiment- all theoretical though. The papers I have found have been interesting though. So time to keep reading!

Comments Off

Apr 18 2011

Genome Estimation

Published by under Uncategorized

After analyzing the second restriction digest I ran, I’ve come up with an estimate for the genome that is around 40,000 bp in length.  The different enzymes, both BAMHI and CLA provided estimates within this range and the prior restriction gel run provided an estimate around 41,000 bp.  The only cluster found within this range was already tested and found to not match BoiC, but new clusters were recorded in the database.  One of these, cluster N, seemed to match this genome size.

Comments Off

Apr 18 2011

SIRC

Published by under Uncategorized

This weekend at the presentation I had a lot of fun. It was a lot less formal than I expected so that made it easier for me to talk to people about our phage research. I met some really cool people and the other students there had done some interesting research themselves. Im really glad I got to participate in the conference, it was a good experience. I also made a connection with a lady at Spokane Community College who teaches microbiology and she was going to send us another strain of mycobacterium to work with. Way cool!

Comments Off

Apr 18 2011

Weekly Update

Published by under Uncategorized

Hello,
Time to update y’all really quick. I still don’t have my primers, but that’s because the first step in my project is to actually grow the bacterial cells that contain the plasmid I am using, pet45b (with HGMR gene between BamHI and HindIII). The bacterial cells are strep and have something altered so that they look blue, which is really cool I think. But anyway, last friday I started my own culture of these cells by making a smear plate and incubating it overnight at 37 C, which worked but not fantastically well. I also made 4x 3mL cultures of LB buffer + .5 microliters of Amp antibiotic. I also shook these overnight at 37 C, and then once my plates were done incubating I swabbed them with a micropipette tip and and mixed the cells into the cultures, again shaking them overnight at 37 C. Today, I am going to extract the plasmid, hopefully, by centrifuging the tubes and collecting the plasmid from the dead cells.
More later,
Peace

Comments Off

Apr 18 2011

Research Update

Published by under Uncategorized

Finally got the results of my PCR back.  Sherri apparently falls into the H1 cluster.  I started looking into other H1 phages restriction digests–they look nothing alike.  For this cluster type, it seems to be that restriction digests aren’t a good method for predicting the cluster type of an individual phage.

Comments Off

Apr 18 2011

SIRC Success!

Published by under Uncategorized

Chelsea and I have successfully given our presentation. We split up the presentation so that she talked about positional analysis and I talked about functional analysis. Overall, I think that we presented very well. I will now move on to start working on my research proposal and my research project.

Comments Off

Apr 18 2011

Bacteria

Published by under Uncategorized

Hopefully I will have some bacteria by next week!!!

Comments Off

Next »

Featuring WPMU Bloglist Widget by YD WordPress Developer